We further estimated absolute copy numbers for genomic segments and annotated genes in each individual. PCR (qPCR), and fluorescent in situ hybridization (FISH), achieving a validation rate of 82% and a false positive rate ofĨ%. We validated this sequence-based CNV call set with array comparative genomic hybridization (aCGH), quantitative Within mappedĬhromosomal sequence, we identified 1265 CNV regions comprising ∼55.6-Mbp sequence-476 of which (∼38%) have not previouslyīeen reported. Using a read depth approach based on next-generation sequencing, we examined genome-wide copy numberĭifferences among five taurine (three Angus, one Holstein, and one Hereford) and one indicine (Nelore) cattle. ↵ 9 Present address: Department of Computer Engineering, Bilkent University, Ankara 06800, Turkey.Ĭopy number variations (CNVs) affect a wide range of phenotypic traits however, CNVs in or near segmental duplication regionsĪre often intractable. 7Howard Hughes Medical Institute, Seattle, Washington 98195, USA.6UNESP-Univ Estadual Paulista, Rua Clóvis Pestana, 793, Araçatuba, SP, Brazil. ![]() 5Division of Animal Sciences, University of Missouri, Columbia, Missouri 65211, USA.4Department of Genetics and Microbiology, University of Bari, Bari 70126, Italy.3Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington 98195, USA. ![]() ![]()
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